Johne’s Test

Lab Services

Johne’s Test

Johne’s Disease is an incurable wasting disease. It is an infectious bacterial disease of livestock that primarily affects the intestinal tract. It is spread from one animal to another in manure-tainted food or water. The microbe can live in manure and/or water for over a year.


  • One in 10 animals moving through livestock auction facilities has Johne’s Disease*
  • Eight out of 100 U.S. beef herds are estimated to be infected with this devastating disease*
  • 68.1% of U.S. dairy operations are infected with Mycobacterium avium paratuberculosis (MAP), the bacteria known to cause Johne’s Disease*

*National Institute of Agriculture.

Testing Method:

The laboratory uses a USDA approved ELISA (enzyme-linked immunosorbent assay) for the description of Mycobacterium paratuberculosis-specific antibodies in serum and milk of cattle.
The sensitivity and specificity performance, as well as the suitability for standardization of the ELISA technique, make it a preferred serologic method for antibody screening.

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